Cyclooxygenase‐catalyzed formation of 9‐hydroxylinoleic acid by guinea pig alveolar macrophages under non‐stimulated conditions

FEBS Letters

Willems, H.¹  Engels, F.¹  Nijkamp, F.P.¹ 

[1] nstitute of Veterinary Pharmacology, Pharmacy, and Toxicology, Department of Pharmacology, State University of Utrecht, PO Box 80176, 3508 TD Utrecht, The Netherlands

关键词: Alveolar macrophage;    9-Hydroxylinoleic acid;    Cyclooxygenase;    Enzyme activity;    Indomethacin;   

DOI  :  10.1016/0014-5793(86)81121-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 × 10⁶ AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), a byproduct of the formation of thromboxane A₂. 9-HODE and HHT were the most prominent products formed under the incubation conditions used. These products were formed via a Cyclooxygenase-catalyzed reaction, since their formation was inhibited by 1 μM indomethacin. Thus, under basal conditions AM possess substantial cyclooxygenase activity. The biological significance of 9-HODE and related substances is discussed.

【 授权许可】

Unknown