Plasminogen activator inhibitor type‐1 : reactive center and amino‐terminal heterogeneity determined by protein and cDNA sequencing

FEBS Letters

Blasi, F.⁴  Danø, K.³  Sartorio, R.³  Douglas, R.¹  Nielsen, L.S.³  Riccio, A.⁴  Oppenheimer, C.¹  Andreasen, P.A.³  Welinder, K.G.² 

[1] Integrated Genetics Inc., 31 New York Avenue, Framingham, MA 01701, USA;Institute of Biochemical Genetics, 2A Øster Farimagsgade, 1353 Copenhagen K, Denmark;Finsen Laboratory, Rigshospitalet, 49 Strandboulevarden, 2100 Copenhagen Ø, Denmark;International Institute of Genetics and Biophysics CNR, 10 Via Marconi, 80123 Naples, Italy

关键词: Plasminogen activator;    Plasminogen activator inhibitor;    Amino acid sequence;    Nucleotide sequence;    Serpin;    PAI-1;    type-1 plasminogen activator inibitor;    PAI-2;    type-2 plasminogen activator inhibitor;    u-PA;    urokinase-type plasminogen activator;    t-PA;    tissue-type plasminogen activator;    PBS;    0.01 M sodium phosphate;    pH 7.4;    0.15 M NaCl;    PAGE;    polyacrylamide gel electrophoresis;    SSC;    0.15 M NaCl;    0.015 M sodium citrate;    pH 7.6;    Denhardt's solution;    0.02Ficoll;    0.02polyvinylpyrrolidone;    0.02bovine serum albumin;    DTT;    dithiothreitol;    PTH;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(86)81113-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Both the urokinase-type and tissue-type plasminogen activator can convert their 5̃4 kDa type-1 inhibitor (PAI-1) to an inactive form with a lower apparent molecular mass. We have determined the amino-terminal amino acid sequences of human native and converted PAI-1, and isolated PAI-1 cDNA and determined the nucleotide sequence in regions corresponding to the amino-terminus and the cleavage site. The data show that the conversion of the inhibitor consists of cleavage of an Arg-Met bond 33 residues from the carboxy-terminus, thus localizing the reactive center of the inhibitor to that position. In addition, a heterogeneity was found at the amino-terminus, with a Ser-Ala-Val-His-His form and a two-residue shorter form (Val-His-His-) occurring in approximately equal quantities.

【 授权许可】

Unknown