The acute GH action in rat adipocytes is associated with enhanced phosphorylation of a 46 kDa plasma membrane protein enriched by GH‐Sepharose

FEBS Letters

Belfrage, Per¹  Donnér, Jakob¹  Eriksson, Hans¹ 

[1] Department of Physiological Chemistry, University of Lund, PO Box 94 S-221 00 Lund, Sweden

关键词: Somatotropin Lipolysis Plasma membrane Phosphorylation;    BSA;    bovine serum albumin;    hGH;    human growth hormone;    PAGE;    polyacrylamide gel electrophoresis;    PMSF;    phenylmethanesulfonyl fluoride;   

DOI  :  10.1016/0014-5793(86)81030-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The acute anti-lipolytic effect of human growth hormone (hGH) in maximally noradrenaline-stimulated intact rat adipocytes was selectively associated with increased phosphorylation of a 46 kDa plasma membrane protein which was highly enriched by hGH-Sepharose chromatography. The same protein was also phosphorylated by an endogenous protein kinase in isolated plasma membranes, although then no hGH effect could be demonstrated. About 14% of the phosphate incorporated into the protein in isolated plasma membranes was found in tyrosine residues and the remainder in serine and threonine. The possible relation of the 46 kDa protein with the hGH plasma membrane receptor is discussed.

【 授权许可】

Unknown