Immunochemical characterization of charge isomers of bacteria‐derived human growth hormone with monoclonal antibodies

FEBS Letters

Ekre, Hans-Peter T.¹  Perlmann, Peter²  Skoog, Bo¹  Jónsdóttir, Ingileif² 

[1] Research Department, Biochemistry and Analytical Chemistry, KabiVitrum AB, S-112 87 Stockholm, Sweden;Department of Immunology, University of Stockholm, S-106 91 Stockholm, Sweden

关键词: Human growth hormone;    Charge isomer;    Monoclonal antibody;    Immunoblotting;    ELISA;    BSA;    bovine serum albumin;    ELISA;    enzyme-linked immunoadsorbent assay;    GαMIg-ALP;    alkaline phosphatase conjugated goat anti-mouse immunoglobulin;    hGH;    human growth hormone;    IEF;    isoelectric focusing;    Mab;    monoclonal antibody;    met-hGH;    bacterially produced methionyl hGH;    PBS;    phosphate-buffered saline;    r.t;    room temperature;   

DOI  :  10.1016/0014-5793(84)80823-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibodies were used to study the immunochemical nature of charge isomers of bacterially produced methionyl human growth hormone. After isoelectric focusing of the hormone the 12 monoclonal antibodies reacted similarly in immunoblotting experiments and none of them could discriminate between the two isolated charge isomers in ELISA. This indicates that the generation of charge isomers of met-hGH does not result is loss of the determinants recognized by the monoclonal antibodies and that the conformation of the two main charge isomers is identical within these determinants.

【 授权许可】

Unknown