A new monoclonal antibody recognizing the amino‐terminal consensus sequence of vertebrate intermediate filament proteins

FEBS Letters

Huc, Claude¹  Boitard, Christian³  Escurat, Michel¹  Bach, Jean-François³  Gros, François¹  Phamgia, Hai⁴  Portier, Marie-Madeleine¹  Pouplard-Barthelaix, Annick² 

[1] Collège de France, Laboratoire de Biochimie Cellulaire, 75 231 Paris Cedex 05, France;INSERM U 298, Centre Hospitalo-Universitaire, Angers France;INSERM U 25, Hôpital Necker, Paris, France;INSERM U 188, Hôpital Saint Vincent de Paul, Paris, France

关键词: Intermediate filament protein;    Monoclonal antibody;    Immunoblotting;    Consensus sequence;    Peripherin;    Neurofilament;   

DOI  :  10.1016/0014-5793(89)80950-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mouse monoclonal antibody ME 101 raised against human peripherin, an intermediate filament protein (IFP) specific to well defined neuronal populations, recognizes all the major classes of vertebrate IFP in immunoblotting assays. Desmin, GFAP, vimentin, peripherin and the lightest neurofilament protein (NF-L) were cleaved into carboxy- and amino-terminal halves by N-chlorosuccinimide at their unique trytophan residue. Whereas the antibody directed against the epitope common to every IFP (intermediate filament antigen or IFA) and located on the carboxy-terminal end of the rod domain recognizes the carboxy-terminal half, the ME 101 antibody, as the present study illustrates, recognizes specifically the amino-terminal half. From the amino acid sequence data of IFP, it is deduced that the cognate epitope is localized on the amino-terminal part of coil la.

【 授权许可】

Unknown