Sliding‐end‐labelling

FEBS Letters

Matallana, Emilia¹  Pérez-Ortin, Josh E.¹  Franco, Luis¹  Estruch, Francisco¹ 

[1] Department of Biochemistry, Faculties of Sciences, University of Valencia, 46100 Burjassot, Valencia, Spain

关键词: Chromatin Nucleosome positioning Indirect end labelling SUC2 gene (Saccharomyces cerevisiae);    MNase;    micrococcal nuclease;    bp;    base pairs;   

DOI  :  10.1016/0014-5793(86)81525-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A method, termed ‘sliding-end-labelling’, has been devised to avoid a frequent artifact in nucleosome positioning by indirect end labelling, namely the appearing of DNA fragments originated by two nuclease cuts, one of them lying within the region covered by the probe. The method is applied to the nucleosome positioning in the yeast SUC2 gene for invertase.

【 授权许可】

Unknown