The excimer fluorescence of N‐(1‐pyrenyl)iodoacetamide labeled to myosin and its subfragment 1

FEBS Letters

Ikkai, Takamitsu¹  Mihashi, Kohsin¹ 

[1] Aichi Prefectural University of Fine Arts, Nagakute, Aichi 480-11 and Department of Physics, Nagoya University, Chikusaku, Nagoya, Japan

关键词: N-(1-Pyrenyl)iodoacetamide;    Excimer;    Myosin;    Subfragment 1;    ATP;    S1;    chymotryptic subfragment 1 of myosin;    PIAA;    N-(1-pyrenyl)iodoacetamide;    DTT;    dithiothreitol;    ATP;    adenosine 5'-triphosphate;    EDTA;    ethylenediaminetetraacetic acid;    Tris-HCl;    tris(hydroxymethyl)aminomethane hydrochloride;   

DOI  :  10.1016/0014-5793(86)80035-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Myosin and its subfragment 1 were labeled with the fluorescent probe N-(1-pyrenyl)iodoacetamide. Both of the labeled complexes exhibited the excimer band at 480 nm (pH 8.0, 25 °C). SH₁ and SH₂ are labeled with this probe as judged by Ca²⁺-ATPase of the labeled complex. Excimers arise both from the interaction of PIAAs in the two different heads within a single myosin molecule and also from the interaction of PIAAs in the same head. ATP affects these excimers depending on the concentration of Ca²⁺.

【 授权许可】

Unknown