Purification of the enhancing factor from mouse intestines

FEBS Letters

Deo, M.G.¹  Wagle, Archana¹  Mulherkar, Rita¹  Saraf, Anjali¹ 

[1] Cancer Research Institute, Tata Memorial Centre, Parel, Bombay 400012, India

关键词: Growth factor;    HPLC;    Receptor modulation;   

DOI  :  10.1016/0014-5793(86)80028-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A unique polypeptide, called enhancing factor (EF), which enhances the binding of labeled epidermal growth factor (EGF) to cells, has been isolated. It has been purified to homogeneity from the acid-soluble proteins of mouse intestines. Earlier, EF was partially purified by two cycles of gel-permeation chromatography on Bio-Gel columns. We now report the final purification of EF on high-performance liquid chromatography (HPLC), using a reverse-phase column (μBondapak C18). The purity of the protein was confirmed when a single peak was obtained in HPLC. Also, a single protein band was obtained in SDS-PAGE. Purified EF has the same properties in vitro as those reported earlier for partially purified EF.

【 授权许可】

Unknown