Acetylcholine stimulates phosphatidylinositol turnover at nicotinic receptors of cultured myotubes

FEBS Letters

Nervi, Clara¹  Zani, Bianca Maria¹  Adamo, Sergio¹  Eusebi, Fabrizio¹  Molmaro, Mario¹  Senni, Maria Immacolata¹ 

[1] Institute of Histology and General Embryology. University of Rome, via A. Scarpa 14,00161 Rome, Italy

关键词: Acetylcholine;    Nicotinic receptor;    Phosphoinositide;    Cultured myotube;    Receptor modulation;   

DOI  :  10.1016/0014-5793(85)80449-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acetylcholine treatment of [³H]inositol pre-labelled cultured chick embryo myotubes results in the stimulation of phosphatidylinositol breakdown, as shown by the measurement of inositol-1-phosphate accumulating in the presence of lithium. The described effect is dependent on agonist concentration and incubation time, and is inhibited by tubocurarine and α-bungarotoxin. The activation of phosphatidylinositol breakdown by acetylcholine at extrajunctional nicotinic receptors is likely to be involved in the modulation of the functional activity of the receptor.

【 授权许可】

Unknown