Protein kinase C translocation in relation to proliferative state of C3H 10T1/2 cells

FEBS Letters

Trawicki, Witold¹  Przybyszewska, Małgorzata²  Moraczewsk, Jerzy¹  Miłoszewska, Joanna²  Janik, Przemysław²  Szaniawska, Bożena² 

[1] Department of Cytology, University of Warsaw, Warsaw, Poland;Department of Tumor Biology, Cancer Center, M. Skłodowska-Curie Institute, Wawelska 15, Warsaw 00-973 Poland

关键词: Protein kinase C;    Proliferation;    Contact inhibition;   

DOI  :  10.1016/0014-5793(86)80997-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinase C (PKC) activity of cytosol and membrane fractions of 10T cells was studied. In cytosol of fast growing cells PKC activity was found in material eluted with 0.150 M NaCl whereas in membrane fractions activity was eluted with 0.065 and 0.150 M NaCl. In the membrane fraction of confluent cells, in contrast to cytosol, very low PKC activity was observed. The translocation pattern of PKC activity eluted with 0.065 M NaCl may be associated with proliferation.

【 授权许可】

Unknown