Site of action of a halogenated 4‐hydroxypyridine on ferredoxin‐catalysed cyclic photophosphorylation

FEBS Letters

Bendall, Derek S.³  Ridley, Stuart M.¹  Patel, Pravin K.² 

[1]ICI Plant Protection Division, Jealott's Hill Research Station, Bracknell RG12 6EY, England

[2]Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 USA

[3]IQW Plant Protection Division, Jealott's Hill Research Station, Bracknell RG12 6EY, England

关键词: Cyclic photophosphorylation;    Hydroxypyridine;    Antimycin;    Q-cycle;    Ferredoxin;    Anthraquinonesulfonate;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    AQS;    9;    10-anthraquinone-2-sulphonate;    J820;    tetrabromo-4-hydroxypyridine;    P518s;    slow phase of the electrochromic shift;    QR site;    quinone reduction site;    QO site;    quinol oxidation site;    DBMIB;    2;    5-dibromo-3-methyl-6-isopropyl-p-benzoquinone;    DNPINT;    2-iodo-6-isopropyl-3-methyl-2 ';    4;    4 '-trinitrodiphenyl ether;    HQNO;    2-heptyl-4-hydroxyquinoline N-oxide;    NQNO;    2-nonyl-4-hydroxyquinoline N-oxide;   

DOI  :  10.1016/0014-5793(86)80990-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tetrabromo-4-hydroxypyridine (J820) inhibited ferredoxin-catalysed cyclic photophosphorylation at micromolar concentrations but did not inhibit or uncouple the AQS-catalysed system. At 2 μM it did not abolish the slow phase of the electrochromic shift or affect the turnover of cytochromes b-563 and f. At higher concentrations (10 μM) it decreased the rate of re-reduction of cytochrome f, whilst inhibiting the reduction of cytochrome b-563. It is concluded that tetrabromo-4-hydroxpyridine does not bind to the quinone reduction site of the cytochrome bf complex, but inhibits the putative ferredoxin-plastoquinone reductase.

【 授权许可】

Unknown