Cytochrome c‐549 ‐ an endogenous cofactor of cyclic photophosphorylation in the cyanobacterium Anacystis nidulans?

FEBS Letters

Peschek, Günter A.¹  Kienzl, Peter F.¹ 

[1] Biophysical Chemistry Group, Institute of Physical Chemistry, University of Vienna, Währingerstraβe 42, A-1090 Vienna, Austria

关键词: Low potential c-type cytochrome;    Cytochrome c-549;    Cyclic photophosphorylation;    Ferredoxin Redox poising;    Anacystis nidulans;    Cyanobacteria;    cyt;    cytochrome;    chl;    chlorophyll;    Fd;    ferredoxin;    PMS;    phenazine methosulfate;    DSPD;    disalicylidene-1;    3-diaminopropane;    DCMU;    3-(3′;    4′-dichlorophenyl)-1;    1-dimethylurea;    DBMIB;    2;    5-dibromo-3-methyl-6-isopropyl-p-benzoquinone;    EDAC;    1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    Hepes;    N-hydroxy-ethylpiperazine-N'-2-ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(83)81052-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cyanobacterium Anacystis nidulans synthesizes fairly large amounts of low potential cytochrome c-549 (E′_o = −260 mV) during late logarithmic and stationary growth phases. We show here that isolated cytochrome c-549 catalyzed high rates of cyclic photophosphorylation by membrane preparations of A. nidulans when added in about 50% reduced state; fully oxidized or reduced cytochrome c-549 was far less efficient. A similarly decisive role of proper redox poising was documented with ferredoxin as the cofactor of cyclic photophosphorylation. Inhibitor studies indicated that plastoquinone and cytochrome f are involved in cyclic electron flow catalyzed by cytochrome c-549 and ferredoxin.

【 授权许可】

Unknown