Changes in the expression of cell surface sialoglycoproteins during transition of human monocytes into macrophages

FEBS Letters

Heino, Jyrki¹  Larjava, Hannu²  Penttinen, Risto¹ 

[1] Department of Medical Chemistry, University of Turku, Kiinamyllynkatu 10, SF-20520 Turku, Finland;Department of Periodontology, University of Turku, Kiinamyllynkatu 10, SF-20520 Turku, Finland

关键词: Glycoprotein;    Plasma membrane;    Monocyte;    Macrophage;    (Synovium);   

DOI  :  10.1016/0014-5793(86)80984-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell surface sialoglycoproteins of human mononuclear phagocytes in different maturation stages were labelled by the periodate/borohydride method and separated by SDS-polyacrylamide gel electrophoresis. The main surface glycoproteins of peripheral blood monocytes had molecular masses of 115 and 95 kDa. During in vitro transition into adherent macrophages, the monocyte-characteristic surface glycoproteins disappeared. Most of the changes in the surface glycoprotein pattern occurred during the first 24 h and after 96 h the changes were completed. The major sialoglycoproteins of the macrophage cell surface had molecular masses of 130 and 55 kDa. The macrophage cell surface showed further changes when cultured in the presence of synovial fluid (10%). These results may reflect the in vivo maturation of monocytes into tissue macrophages. In synovium, tissue-derived factors may also take part in differentiatíon.

【 授权许可】

Unknown