Structure‐linked latency of muscarinic receptors in axonal transport

FEBS Letters

Laduron, Pierre M.¹  Scholtus, Thierry¹ 

[1]Department of Biochemical Pharmacology, Janssen Pharmaceutica, Turnhoutseweg 30, B-2340 Beerse, Belgium

关键词: Axonal transport;    Muscarinic receptor;    Latency;    Synaptic vesicle N-Methylscopolamine;   

DOI  :  10.1016/0014-5793(86)81345-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Muscarinic receptors that accumulated above a ligature in rat sciatic nerves were labelled in vitro under isotonic conditions withN[³H]methylscopolamine. The addition of 0.005% of digitonin doubled the binding in proximal segments above and close to the ligature but not in the intermediary segments between two ligatures. Osmotic shock and freeze-thawing treatments also enhanced the binding. Digitonin did not affect the affinity of muscarinic receptors but revealed a greater number of sites by increasing the membrane permeability to the hydrophilic ligand. We conclude that presynaptic muscarinic receptors that undergo fast axonal transport in rat sciatic nerves exist under a latent form because they are associated with vesicles. This is the first demonstration of a structure-linked latency for receptors.

【 授权许可】

Unknown