Chilling followed by incubation at 37°C causes a reduction in NAD+ levels which can be prevented by the poly(ADP‐ribose)transferase inhibitor 3‐aminobenzamide

FEBS Letters

Cantoni, Orazio¹  Sestili, Piero²  Cattabeni, Flaminio²  Stocchi, Vilberto¹ 

[1] Istituto di Chimica Biologica, Università di Urbino, Italy;Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale Università di Urbino, Italy

关键词: Cold shock NAD+Poly(ADP-ribose)transferase 3-Aminobenzamide DNA damage;    NAD+;    nicotinamide adenine dinucleotide;    ADPRT;    poly(ADP-ribose)transferase;    3-AB;    3-aminobenzamide;    CHO;    Chinese hamster ovary;   

DOI  :  10.1016/0014-5793(86)80825-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The exposure of cells for 60 min to a serum free medium at ice temperature followed by a return to normal culture conditions (30 min at 37°C) caused a dramatic decrease in NAD⁺ levels. This decrease in NAD⁺ was prevented by 3-aminobenzamide. Alkaline elution analysis of DNA from cultures that were sisters to the ones utilized for measuring cellular NAD⁺ content revealed an absence of DNA breakage. These data suggest that poly(ADP-ribose)transferase may be induced in conditions not involving DNA fragmentation. The induction of this enzyme could therefore represent a cellular emergency reaction and not just a response to DNA damage.

【 授权许可】

Unknown