Archaebacterial and eukaryotic ribosomal subunits can form active hybrid ribosomes

FEBS Letters

Cammarano, Piero¹  Altamura, Sergio¹  Londei, Paola¹ 

[1] Dipartimento di Biopatologia Umana, Sezione di Biologia Cellulare, Università di Roma ‘La Sapienza’, Policlinico Umberto I, 00161 Rome, Italy

关键词: Sulphur-dependent archaebacteria;    Ribosomal subunit exchange;    Peptidyltransferase;    Evolution;    Phe;    phenylalanine;    acPhe;    N-acetyl-phenylalanine;    PTF;    peptidyltransferase;   

DOI  :  10.1016/0014-5793(86)81400-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Purified ribosomal subunits from the extremely thermoacidophilic archaebacterium Sulfolobus solfataricus are able to recognize ribosomal subunits from the yeast Saccharomyces cerevisiae forming hybrid monosomes that can be revealed by sucrose gradient analysis and are active in peptide bond formation. Both reciprocal combinations (archaebacterial 30 S + eukaryotic 60S and archaebacterial 50 S + eukaryotic 40 S) are functional. In contrast, no hybrid couples are formed between subunits of yeast and Escherichia coli ribosomes. These results indicate that ribosomes of at least one archaebacterial species share specific structural features with those of the lower eukaryote S. cerevisiae.

【 授权许可】

Unknown