Part of the 23S RNA located in the 11S RNA fragment is a constituent of the ribosomal peptidyltransferase centre

FEBS Letters

Kuechler, Ernst¹  Barta, Andrea¹ 

[1] Institut für Biochemie, University of Vienna, Währingerstr. 17, A-1090 Vienna, Austria

关键词: E. coli ribosome;    Photoaffinity labelling;    Benzophenone derivative;    23S RNA;    Cross-link;    Peptidyltransferase;    BP-PhetRNA;    3-[4-benzoylphenyl]propionyl-PhetRNA;   

DOI  :  10.1016/0014-5793(83)80844-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Upon irradiation, 3-[4-benzoylphenyl]propionyl-PhetRNA bound to the P-site of poly(U)-primed ribosomes is exclusively cross-linked to 23S RNA. It is shown that the photoreaction only occurs with pyrimidine nucleotides. The site of the cross-link is located within an 11S RNA fragment, which comprises the 1100 nucleotides at the 3′-end of 23S RNA. The cross-linked Phe tRNA derivative is still functionally active in peptide bond formation. The site labelled on the 11S fragment is therefore an integral part of the peptidyltransferase centre.

【 授权许可】

Unknown