The primary structure of protein L17 from the Escherichia coli ribosome

FEBS Letters

Feytons, Valère²  Rombauts, Wilfried²  Wittmann-Liebold, Brigitte¹ 

[1] Max-Planck-Institute für Molekulare Genetik, Abteilung Wittmann, Ihnestrasse 63-73, 1000 Berlin 33 (Dahlem), Germany;Afdeling Biochemie, Departement Humane Biologie, Katholieke Universiteit, 49, Herestraat, 3000 Leuven, Belgium

关键词: E. coli ribosome;    Protein L17;    Primary structure determination;    Secondary structure predictions;    Homology to other ribosomal proteins;   

DOI  :  10.1016/0014-5793(82)81124-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete sequence of protein L17 which is a component of the large subunit of the E. coli ribosome has been determined. Peptides deriving from enzymatic hydrolysis with trypsin, thermolysin, chymotrypsin and S. aureus and A. mellea protease were isolated and sequenced by the DABITC/PITC double coupling method. Some overlapping peptides were obtained after mild acid cleavage of the protein. According to the amino acid sequence protein L17 contains 127 residues and has a molecular mass of 14 365. The primary structure of protein L17 agrees well with the amino acid analysis of the intact protein and its N-terminal sequence as derived from automatic sequencing in an improved Beckman sequencer. Secondary predictions and a search for homologous sequence stretches to other ribosomal proteins were made.

【 授权许可】

Unknown