Deficiency of neutrophil membrane antigen detected by monoclonal antibody in rheumatoid arthritis

FEBS Letters

Spisani, Susanna²  Gavioli, Riccardo²  Sensi, Alberto³  Dovigo, Livio¹  Damiani, Guido⁴  Traniello, Serena²  Baricordi, Roberto O.³ 

[1] Divisione di Reumatologia, Arcispedale S. Anna, Corso Giovecca n. 203, 44100 Ferrara,16132 Genova, Italy;Istituto di Chimica Biologica ,16132 Genova, Italy;Genetica Medica dell'Università di Ferrara, via Luigi Borsari 46, 16132 Genova, Italy;Istituto di Chimica Biologica, Viale Benedetto XV, 16132 Genova, Italy

关键词: Neutrophil activation;    Monoclonal antibody;    Rheumatoid arthritis;    Superoxide production;    mAb;    monoclonal antibody;    PMA;    phorbol 12-O-myristate 13-acetate;    RA;    rheumatoid arthritis;    FMLP;    N-formly-methionyl-leucyl-phenyl-alanine;    O/t- 2;    superoxide anion;    KRPG;    Krebs-Ringer-phosphate containing 0.2% () glucose;   

DOI  :  10.1016/0014-5793(86)81385-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibodies (mAbs) against cell surface antigens and receptors are instrumental in defining specific membrane markers. mAbs GF26.7.3 and MF25.1 against human neutrophils modulated the activation mechanism of superoxide anion production induced by formyl-peptide and PMA in all subjects. However, treatment with mAb MF 25.1 binds is absent in rheumatoid conditions. This confirms our previous data showing that defective expression of membrane components is associated with neutrophil dysfunction.

【 授权许可】

Unknown