Impaired production of nitric oxide, superoxide, and hydrogen peroxide in glucose 6‐phosphate‐dehydrogenase‐deficient granulocytes

FEBS Letters

Chao, Sin Sin³  Hung, Iou-Jih¹  Tsai, Kan-Jen³  Chiu, Daniel Tsun-Yee³  Chow, Ching Kwong⁴  Stern, Arnold² 

[1] Department of Hematology, Chang Gung Children's Hospital, Kwei-san, Tao-yuan, Taiwan;Department of Pharmacology, New York University Medical Center, New York, NY, USA;Graduate Institute of Basic Medical Sciences and School of Medical Technology, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-san, Tao-yuan, Taiwan;Department of Nutrition and Food Science, University of Kentucky, Lexington, KY, USA

关键词: G6PD deficiency;    Nitric oxide production;    Superoxide production;    Granulocyte;   

DOI  :  10.1016/S0014-5793(98)01174-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Since the generation of superoxide and hydrogen peroxide by NADPH oxidase and nitric oxide (NO) by NO synthase (NOS) in granulocytes is NADPH-dependent, we investigated the production of NO, superoxide and H₂O₂ in glucose 6-phosphate dehydrogenase (G6PD)-deficient human granulocytes. Our results showed that upon stimulation with either 5 μg/ml of lipopolysaccharide (LPS) or 10 μM of phorbol 12-myristate 13-acetate (PMA), the production of nitrite in normal granulocytes was elevated, 252±135% and 239±72%, respectively, compared to the resting stage. In contrast, G6PD-deficient granulocytes did not produce more nitrite upon stimulation with either LPS or PMA compared to the resting stage. Western blot analysis indicated a normal expression pattern of inducible NOS in G6PD-deficient granulocytes. In addition, the production of H₂O₂ and superoxide was also significantly impaired in G6PD-deficient granulocytes compared to control cells. These data demonstrate that G6PD deficiency causes an impairment in the production of NO, superoxide and H₂O₂.

【 授权许可】

Unknown