Isolation and amino acid sequence of a mating pheromone produced by mating type α cells of Saccharomyces exiguus

FEBS Letters

Takahashi, Nobutaka²  Tanaka, Hiroko²  Hiscatomi, Taisuke¹  Esumi, Yasuaki²  Sakurai, Akira²  Banno, Isao³  Yanagishima, Naohiko¹ 

[1] Biological Institute, Faculty of Science, Nagoya University, Chikusa-ku, Nagoya 464 Japan;The Institute of Physical and Chemical Research, Wako-shi, Saitama 351-01 Japan;Institute for Fermentation, Osaka, Yodogawa-ku, Osaka 532, Japan

关键词: (Saccharomyces exiguus);    Mating pheromone;    αse pheromone;    Amino acid sequence;    HPLC;    high-performance liquid chromatography;    SIMS;    secondary ion mass spectrometry;   

DOI  :  10.1016/0014-5793(86)80759-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A peptide, termed α^se pheromone, was isolated as a mating pheromone from culture filtrate of mating type a cells of Saccharomyces exiguus. The peptide showed both agglutinability-inducing activity to a cells of S. cerevisiae and shmoo-inducing action to a cells of S. cerevisiae, S. kluyveri and S. exiguus. The amino acid sequence of α^se pheromone was determined as H-Trp-His-Trp-Leu-Arg-Leu-Ser-Tyr-Gly-Gln-Pro-Ile-Tyr-OH by mass spectrometry, sequence analysis and enzymatic digestion.

【 授权许可】

Unknown