Isolation and amino acid sequence of a mating pheromone produced by mating type α cells of Saccharomyces kluyveri

FEBS Letters

Takahashi, Nobutaka¹  Tanaka, Hiroko¹  Sakamoto, Mitsuhiro¹  Esumi, Yasuaki¹  Sakurai, Akira¹  Banno, Isao³  Fujimura, Hiroaki²  Yanagishima, Naohiko² 

[1] Institute of Physical and Chemical Research, Wako, Saitama 351, Japan;Biological Institute, Faculty of Science, Nagoya University, Chikusa-ku, Nagoya 464, Japan;Institute for Fermentation, Osaka, Yodogawa-ku, Osaka 532, Japan

关键词: Saccharomyces kuyveri;    Mating pheromone;    αsk pheromone;    Amino acid sequence;    HPLC;    high-performance liquid chromatography;    SIMS;    secondary ion mass spectrometry;   

DOI  :  10.1016/0014-5793(84)80108-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A peptide, termed α^sk2 pheromone, was isolated from culture filtrates of mating type α cells of Saccharomyces kluyveri as a mating pheromone, having both shmoo inducing (in a cells of S. kluyveri and S. cerevisiae) and agglutinability inducing (in a cells of S. cerevisiae) actions. The amino acid sequence of α^sk2 pheromone was determined as H—Trp—His—Trp—Leu—Ser—Phe—Ser—Lys—Gly—Glu—Pro—Met—Tyr-OH by mass spectrometry, sequence analysis and enzymatic digestions.

【 授权许可】

Unknown