FEBS Letters | |
Effect of preillumination on the P‐680+ reduction kinetics in chloride‐free photosystem II membranes | |
Gleiter, Hermann2  Ono, Taka-aki2  Conjeaud, Helene1  Inoue, Yorinao2  Mathis, Paul1  | |
[1] Service de Biophysique, Département de Biologie, Centre d'Études Nucléaires de Saclay, 91191 Gif-sur-Yvette, France;Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan | |
关键词: Oxygen evolution; S-state transition; Cl−; P-680; Thermoluminescence; Photosystem II membrane; PS; photosystem; Mes; 4-morpholine-ethanesulfonic acid; Chl; chlorophyll; QA; primary quinone acceptor of PS II; Qb; secondary quinone acceptor of PS II; | |
DOI : 10.1016/0014-5793(86)80745-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The re-reduction course of P-680+, the photooxidized PS II primary donor, was measured as a function of excitation number in Cl−-depleted PS II membranes. After the 1st and 2nd excitations the signal amplitude of P-680+ is small, indicating a submicrosecond reduction of P-680+ by Z, the secondary donor of PS II. After the 3rd excitation, however, a larger P-680+ signal with a 40–50 μs half-life is observed. The slow decay of this signal is attributed to a back-reaction with a reduced acceptor in the presence of the Z+S2 state on the donor side. The state Z+S2 has a lifetime longer than 300 ms and its formation was found to depend on the presence of the abnormal S2 state created by the 1st excitation. The P-680 data and thermoluminescence measurements show that the S-state advancement beyond S2 is blocked in the absence of Cl− and that the Cl−-free abnormal S2 state has a lifetime about 10-times longer than the normal S2 state.
【 授权许可】
Unknown
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