Promotor and enhancer like activity at the 5'‐end of normal and T24 Ha‐ras1 genes

FEBS Letters

Riggio, Marcello¹  Spandidos, Demetrios A.¹ 

[1] The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G611BD, Scotland

关键词: Ha-ras1 oncogene;    Enhancer;    Promoter;    Enzyme assay;    Chloramphenicol acyltransferase;   

DOI  :  10.1016/0014-5793(86)80736-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used a short-term transfection technique, in which we monitor the ability of DNA fragments to induce the expression of the chloramphenicol acetyltransferase (CAT) gene in rat 208F fibroblast cells. Using appropriate vectors we have assayed for promoter or enhancer activity of the 0.8 kb SstI fragment located within the 5'-flanking sequences of the first coding exon of the human T24 and normal Ha-ras1 genes. We find that this fragment contains promoter and enhancer activities in both the normal and the T24 Ha-ras1 gene.

【 授权许可】

Unknown