Hydroperoxyfatty acids inactivate the reticulocyte lipoxygenase independently of a hydroperoxidase reaction

FEBS Letters

Härtel, B.¹  Schewe, T.¹  Kühn, H.¹  Rapoport, S.¹ 

[1] Institute of Biochemistry, Humboldt University Berlin, Hessische Str. 3-4, DDR-104 Berlin, GDR

关键词: Lipoxygenase;    Enzyme inactivation;    Hydroperoxidase reaction;    Hydroperoxyfatty acid;    9-HPODE;    9-hydroperoxy-10;    12(E;    Z)-octadecadienoic acid;    13-HPODE;    13-hydroperoxy-9;    11(Z;    E)-octadecadienoic acid;   

DOI  :  10.1016/0014-5793(86)80687-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

From a comparison of 9D_s-HPODE and 13L_s-HPODE and their methyl esters as substrates and inactivating agents of reticulocyte lipoxygenase it is concluded that the compounds inactivate the enzyme independently of any hydroperoxidase reaction. The protective effect of 4-nitrocatechol indicates the formation of Fe(III) complexes of the enzyme with the hydroperoxyfatty acid compounds prior to inactivation.

【 授权许可】

Unknown