Monoclonal antibodies against GA13‐imide recognize the endogenous plant growth regulator, GA4, and related gibberellins

FEBS Letters

Eberle, J.¹  Yamaguchi, I.²  Takahashi, N.²  Nakagawa, R.²  Weiler, E.W.¹ 

[1] Pflanzenphysiologie, Fachbereich 5, Universität Osnabrück, Postfach 4469, D 4500 Osnabrück, FRG;Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Gibberellin Monoclonal antibody Plant growth regulator-monoclonal antibody;    BSA;    bovine serum albumin;    GA;    gibberellin A;    HPLC;    high-pressure liquid chromatography;    MAB;    monoclonal antibody;    PGR;    plant growth regulator;    RIA;    radioimmunoassay;   

DOI  :  10.1016/0014-5793(86)80642-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibody(MAB)-secreting hybridomas were selected from fusions of spleen cells derived from Balb/c mice immunized with the BSA conjugate of GA₁₃-19,20-imide-β-alanine 7-methyl ester with the myeloma line X63.Ag8.653. Selection based on [1,2-³H]GA₄ methyl ester binding capacity yielded two stable hybridoma clones that secreted antibodies of the IgGl subclass. These MAB exhibited high affinities for GA₄ methyl ester (3.2 × 10⁸ and 1.0 × 10⁹ M⁻¹) which allowed quantitation by HPLC-RIA of nanogram or sub-nanogram amounts of the gibberellins A₂, A₃, A₄ A₇ and A₉ as the methyl esters, in biological fluids.

【 授权许可】

Unknown