FEBS Letters | |
Monoclonal antibodies against GA13‐imide recognize the endogenous plant growth regulator, GA4, and related gibberellins | |
Eberle, J.1  Yamaguchi, I.2  Takahashi, N.2  Nakagawa, R.2  Weiler, E.W.1  | |
[1] Pflanzenphysiologie, Fachbereich 5, Universität Osnabrück, Postfach 4469, D 4500 Osnabrück, FRG;Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan | |
关键词: Gibberellin Monoclonal antibody Plant growth regulator-monoclonal antibody; BSA; bovine serum albumin; GA; gibberellin A; HPLC; high-pressure liquid chromatography; MAB; monoclonal antibody; PGR; plant growth regulator; RIA; radioimmunoassay; | |
DOI : 10.1016/0014-5793(86)80642-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Monoclonal antibody(MAB)-secreting hybridomas were selected from fusions of spleen cells derived from Balb/c mice immunized with the BSA conjugate of GA13-19,20-imide-β-alanine 7-methyl ester with the myeloma line X63.Ag8.653. Selection based on [1,2-3H]GA4 methyl ester binding capacity yielded two stable hybridoma clones that secreted antibodies of the IgGl subclass. These MAB exhibited high affinities for GA4 methyl ester (3.2 × 108 and 1.0 × 109 M−1) which allowed quantitation by HPLC-RIA of nanogram or sub-nanogram amounts of the gibberellins A2, A3, A4 A7 and A9 as the methyl esters, in biological fluids.
【 授权许可】
Unknown
【 预 览 】
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RO201912020288106ZK.pdf | 507KB | download |