Incorporation of [3H]glucosamine into keratin‐related polypeptides in pig epidermis

FEBS Letters

King, Ian A.¹ 

[1] Dermatology Research Group, MRC Clinical Research Centre, Watford Road, Harrow HA1 3UJ, England

关键词: Keratin polypeptide;    Intermediate filament;    Glycoprotein synthesis;    Explant culture;    (Pig epidermis);   

DOI  :  10.1016/0014-5793(86)80581-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Metabolic labelling studies have provided evidence for glycosylated keratins in cultured pig epidermis. [³H]Glucosamine was incorporated into five major particulate polypeptides of M _r 68000, 61000, 57000, 53 000 and 48 000. Radioactivity was present in protein-bound carbohydrate. Non-enzymic glycation was excluded. Labelling was largely unaffected by tunicamycin indicating that radioactivity was incorporated mainly into O-linked oligosaccharides. These [³H]glucosamine-labelled components were closely related to keratins since (i) they had a similar electrophoretic mobility to polypeptides of purified pig prekeratin, (ii) they were immunoprecipitated by anti-prekeratin serum and (iii) they were incorporated into reconstituted, intermediate-sized, keratin filaments.

【 授权许可】

Unknown