Changes in the expression of N‐acetylglucosaminyltransferase III, IV, V associated with the differentiation of HL‐60 cells

FEBS Letters

Koenderman, Anky H.L.¹  van den Eijnden, Dirk H.¹  Wijermans, Pierre W.¹ 

[1] Department of Medical Chemistry, Vrije Universiteit, Amsterdam, The Netherlands

关键词: N-Acetylglucosaminyltransferase III;    N-Acetylglucosaminyltransferase IV;    N-Acetylglucosaminyltransferase V;    Differentiation induction;    HPLC;    Glycoprotein synthesis;    (HL-60 cell);    GlcNAc;    N-acetylglucosamine;    Man;    mannose;    Ara-C;    cytosine-arabinoside;    DMSO;    dimethyl sulfoxide;    vit D3;    1α;    25-dihydroxyvitamin D3;    HPLC;    high-performance liquid chromatography;    Mes;    2-(N-morpholino) ethanesulfonic acid. Enzymes: UDP-GlcNAc: Manβ1 → 4GlcNAc-R β1 → 4-N-acetylglucosaminyltransferase;    GlcNAc-transferase III (EC 2.4.1.144);    UDP-GlcNAc: Manα1 → 3Man-R β1 → 4-N-acetylglucosaminyltransferase;    GlcNAc-transferase IV (EC2.4.1.145);    UDP-GlcNAc: Manα1 → 6Man-R β1 → 6-N- acetylglucosaminyltransferase;    GlcNAc-transferase V (EC 2.4.1.155);   

DOI  :  10.1016/0014-5793(87)80188-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By use of a new assay method based on HPLC, GlcNAc-transferase III, IV and V activities were determined in HL-60 cells, differentiated HL-60 cells and normal myeloid cells. Differentiation along the monocytic lineage with 1α,25-dihydroxyvitamin D₃ resulted in increased GlcNAc-transferase IV and decreased GlcNAc-transferase III activity. Differentiation along the myeloid lineage with retinoic acid resulted in a decrease in GlcNAc-transferase III activity. Although differentiated HL-60 cells show a changed GlcNAc-transferase pattern, they do not resemble normal myeloid cells. Macrophages and granulocytes are characterized by a very low level of GlcNAc-transferase III activity whereas differentiated HL-60 cells still contain this activity. This is the first demonstration of GlcNAc-transferase IV and V activity in a human cell.

【 授权许可】

Unknown