Phosphorylation of pig brain diacylglycerol kinase by endogenous protein kinase

FEBS Letters

Kanoh, Hideo¹  Ono, Teruo¹ 

[1] Department of Biochemistry, Niigata University school of Medicine, Asahimachi- Dori, 1, Niigata 951, Japan

关键词: Diacylglycerol kinase;    (Rabbit);    Antibody;    Protein phosphorylation;    Protein kinase;   

DOI  :  10.1016/0014-5793(86)80577-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pig brain diacylglycerol kinase did not catalyze autophosphorylation. However, the kinase was phosphorylated on serine, when immunoprecipitated from the partially purified enzyme preparation preincubated with Mg²⁺ and [γ-³²P]ATP. The action of the endogenous protein kinase phosphorylating diacylglycerol kinase was independent of cyclic nucleotides and Ca²⁺, and became maximum at pH 5.5. Although the extent of enzyme phosphorylation was limited (maximally about 0.25 mol P_i incorporated per mol kinase), the results show that diacylglycerol kinase can be a phosphoprotein.

【 授权许可】

Unknown