Characteristics of two types of legumin genes in the field bean (Vicia faba L.var. minor) genome as revealed by cDNA analysis

FEBS Letters

Bassüner, Ronald¹  Heim, Ute¹  Saalbach, Gerhard¹  Weschke, Winfriede¹  Bäumlein, Helmut¹  Wobus, Ulrich¹  Müntz, Klaus¹  Jung, Rudolf¹ 

[1]Zentralinstitut für Genetik und Kulturpflanzenforschung, GDR Academy of Sciences, DDR-4325 Gatersleben, GDR

关键词: DNA sequence analysis;    storage protein gene;    (Vicia faba);    Legumin;    Gene subfamily;   

DOI  :  10.1016/0014-5793(86)80573-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nucleotide sequence analysis of cDNA clones coding for field bean legumin precursor polypeptides revealed two different types, called A and B. Although homologous, both types differ in several sequence characteristics. Comparison with similar data from soybean and recent findings from pea leads to the following conclusions: (i) the two types of legumin genes described represent two subfamilies, A and B, which are probably of widespread occurrence; (ii) legumin genes or subunits can best be placed in either subfamily A or B by sequence homology, in addition B-type subunits contain generally fewer (or none at all in V. faba) Met residues as compared to A-type subunits; (iii) members of one subfamily from different species are more homologous than members of either subfamily within a species, therefore the two subfamilies must have arisen long before speciation of the genera Glycine, Pisum and Vicia; (iv) during speciation members of the B-subfamily diverged significantly more than members of the A-subfamily.

【 授权许可】

Unknown