Lipoprotein‐binding proteins in the human platelet plasma membrane

FEBS Letters

Koller, Elisabeth¹ 

[1] Institut für Medizinische Physiologie, Universität Wien, Schwarzspanierstr. 17, A-1090 Wien, Austria

关键词: Platelet Plasma membrane Lipoprotein receptor Ligand blotting Modification;    VLDL;    very low density lipoproteins;    LDL;    low density lipoproteins;    HDL;    high density lipoproteins;    PRP;    platelet-rich plasma;    ACD blood;    acid-citrate-dextrose blood;    PBS;    phosphate-buffered saline;    NC;    nitrocellulose;    FITC;    fluorescein isothiocyanate;    DPH;    1;    6-diphenyl-1;    3;    5-hexatriene;    CHD;    1;    2-cyclohexanedione;    TNBS;    2;    4;    6-trinitrobenzenesulfonic acid;   

DOI  :  10.1016/0014-5793(86)80518-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of homologous plasma lipoproteins to specific receptor proteins in the plasma membrane of human blood platelets was studied by ligand blotting techniques. HDL₃, HDL₂ and LDL showed saturable binding to three bands of 156, 130 and 115 kDa, respectively. This binding was not markedly affected by the presence or absence of Ca²⁺ nor by covalent modification of lysine and arginine residues of the apoprotein moieties. However, it can be almost completely reversed by the addition of heparin or suramin.

【 授权许可】

Unknown