The occurrence and distribution of α‐hydroxy‐acid dehydrogenase in some members of the order Kinetoplastida

FEBS Letters

Gutteridge, Winston E.¹  Taylor, Mark B.¹ 

[1] Biological Laboratory, University of Kent, Canterbury CT2 7NJ, England

关键词: Lactate dehydrogenase;    Gossypol;    Microbody;    Glycosome;   

DOI  :  10.1016/0014-5793(86)80487-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

LDH-X is the isoenzyme of lactate dehydrogenase found in mammalian spermatozoa, occurring in cytosolic and mitochondrial locations. Gossypol strongly inhibits it, and the spermicidal action of this compound is attributed to the disruption of a reducing shuttle. The flagellated protozoan, Trypanosoma cruzi, contains an enzyme activity similar to LDH-X, called α-hydroxy-acid dehydrogenase, which is here shown to possess cytosolic and glycosomal components. The glycosome is a microbody-like organelle containing the early glycolytic enzymes. We postulate that the inhibition of replication of T. cruzi by gossypol derives from interference with glycosomal reducing shuttles. T. lewisi resembles T. cruzi in this respect.

【 授权许可】

Unknown