Localization of the initial steps in alkoxyphospholipid biosynthesis in glycosomes (microbodies) of Trypanosoma brucei

FEBS Letters

Opperdoes, Fred R.¹ 

[1] Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, Avenue Hippocrate 74, B-1200 Brussels, Belgium

关键词: Alkoxyphospholipid;    Glycerol-ether lipid;    Dihydroxyacetone-phosphate acyltransferase;    Acyl-CoA reductase;    Trypanosoma;    Microbody;    Glycosome;    DHAP;    dihydroxyacetone phosphate;    G-3-P;    sn-glycerol 3-phosphate;    CoA;    coenzyme A;   

DOI  :  10.1016/0014-5793(84)80284-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell fractionation of Trypanosoma brucei cultured procyclic stages showed that the key enzyme of glycerol-ether lipid synthesis, dihydroxycetone-phosphate acyltransferase (EC 2.3.1.42) was exclusively associated with the microbody fraction. These organelles contained in addition 1-acyl glycerol-3-phosphate:NADP⁺ oxidoreductase (EC 1.1.1.101) and acyl-CoA reductase and were capable of utilizing DHAP, but not G-3-P, as substrate for lysophosphatidic acid formation. It is concluded that in T. brucei the glycosomes are the exclusive site of the synthesis of precursors for glycerol-ether lipid synthesis and that they contain the entire pathway to form alkoxylipids from glycerol and acyl-CoA.

【 授权许可】

Unknown