Effects of cholesterol on the kinetics of mitochondrial ATPase

FEBS Letters

Solaini, G.²  Rindina, F.Calanni²  Castelli, G.Parenti²  Rabbi, A.²  Baracca, A.¹ 

[1] Istituto ed Orto Botanico Universita di Bologna 40126 Bologna, Italy;Istituto di Chimica Biologica Universita di Bologna 40126 Bologna, Italy

关键词: ATP hydrolysis;    Enzyme kinetics;    Proton translocation;    Cholesterol;    Temperature effect;    Mitochondria;   

DOI  :  10.1016/0014-5793(86)80435-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Enrichment of the inner mitochondrial membrane with cholesterol induces an increase in ATPase activity with a decrease in the K _m for ATP. Cholesterol also abolishes the discontinuity normally found in the Arrhenius plot of ATPase activity. Since no change is detected in the rate of proton translocation through the ATPase membrane sector, it is concluded that cholesterol incorporation induces changes in the hydrolytic step of ATPase via a conformational change transmitted from the membrane sector to the catalytic sector F₁.

【 授权许可】

Unknown