Interaction of actin with dansyl‐tropomyosin

FEBS Letters

Bhangu, K.¹  Burtnick, L.D.¹ 

[1] Department of Chemistry, University of British Columbia, Vancouver V6T 1Y6, British Columbia, Canada

关键词: Actin;    Tropomyosin;    Fluorescence polarization;    Polymerization;    TM;    tropomyosin;    DNS;    dansyl;    dimethylaminonaphthalenesulfonyl;    DTT;    DL-dithiothreitol;   

DOI  :  10.1016/0014-5793(86)80426-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

5-[Dimethylamino]naphthalene-1-sulfonyl chloride (dansyl chloride) reacts with rabbit skeletal muscle tropomyosin (TM) to yield a highly fluorescent product, DNS-TM. The extent of modification of TM can be regulated over a wide range, 0.3–15.5 dansyl groups per TM, depending upon the temperature and duration of the reaction. However, under all conditions employed, about 15 different fluorescent tryptic peptides of TM were produced. DNS-TM undergoes end-to-end polymerization at low ionic strengths, but to a somewhat lesser extent than unlabelled TM does. DNS-TM also binds muscle F-actin. This interaction may be followed fluorimetrically by observing a blue-shift in emission maximum, an increase in fluorescence intensity or an increase in fluorescence polarization of the DNS-TM complex with F-actin.

【 授权许可】

Unknown