Micro-scale temperature measurement method using fluorescence polarization

7th European Thermal-Sciences Conference

Tatsumi, K.^1 ; Hsu, C.-H.^1 ; Suzuki, A.^1 ; Nakabe, K.^1

Department of Mechanical Engineering and Science, Kyoto University, Kyotodaigakukatsura, Nishikyo-ku, Kyoto, Kyoto

6158540, Japan^1

关键词: Basic characteristics;    Fluid temperatures;    Fluorescence polarization;    Fluorescent molecules;    Measurement techniques;    Microscopic scale;    Temperature measurement methods;    Theoretical values;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/745/3/032097/pdf
DOI  :  10.1088/1742-6596/745/3/032097

来源: IOP

【 摘 要 】

A novel method that can measure the fluid temperature in microscopic scale by measuring the fluorescence polarization is described in this paper. The measurement technique is not influenced by the quenching effects which appears in conventional LIF methods and is believed to show a higher reliability in temperature measurements. Experiment was performed using a microchannel flow and fluorescent molecule probes, and the effects of the fluid temperature, fluid viscosity, measurement time, and pH of the solution on the measured fluorescence polarization degree are discussed to understand the basic characteristics of the present method. The results showed that fluorescence polarization is considerably less sensible to these quenching factors. A good correlation with the fluid temperature, on the other hand, was obtained and agreed well with the theoretical values confirming the feasibility of the method.