Null and electrophoretic mobility mutants in the structural gene for L‐lactate dehydrogenase of Saccharomyces cerevisiae

FEBS Letters

Sbragia, Ralph¹  Wills, Christopher¹  Rush, Karen¹ 

[1] Department of Biology, C-016, University of California, San Diego, La Jolla, CA 92093, USA

关键词: Activity staining;    Structural gene mutation;   

DOI  :  10.1016/0014-5793(86)81190-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A mutant lacking L-lactate dehydrogenase (EC 1.1.2.3) of Saccharomyces cerevisiae was isolated by its inability to grow on minimal medium with L-lactate as a carbon source. A simple activity gel assay for visualization of this enzyme and the two D-lactate dehydrogenases in this organism (EC 1.1.2.4 and 1.1.1.28) was developed. This enabled us to screen spontaneous and ethylmethanesulfonate-induced back mutants for electrophoretic mobility. Two mutants with a mobility faster than that of the wild type were isolated, and proved to be allelic to the L-lactate dehydrogenase negative mutant.

【 授权许可】

Unknown