Direct immunological identification of full‐length cDNA clones for plant protein without gene fusion to E. coli protein

FEBS Letters

Nakamura, Kenzo¹  Asahi, Tadashi¹  Hattori, Tsukaho¹ 

[1] Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan

关键词: cDNA;    Immunological screening;    Direct expression;    Patatin;    (Potato tuber);   

DOI  :  10.1016/0014-5793(86)81175-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By immunological screening of a cDNA library constructed from potato tuber poly(A)⁺ RNA and Escherichia coli expression vector pUC8 by the vector-primer and linker procedure of Okayama and Berg [(1982) Mol. Cell Biol. 2, 161-170], nearly full-length cDNA clones for patatin, a major protein of potato tuber, were identified. The cDNA carrying part of the 5'-noncoding region of the patatin mRNA, in addition to entire coding and 3'-noncoding regions, expressed prepatatin in E. coli cells by translational initiation inside cDNA. These results suggest that nearly full-length cDNA clones with entire coding region can be identified directly by immunological screening without gene fusion to E. coli proteins at least for some plant mRNAs.

【 授权许可】

Unknown