Coding sequences for chloroplast ribosomal protein S12 from the liverwort, Marchantia polymorpha, are separated far apart on the different DNA strands

FEBS Letters

Umesono, Kazuhiko¹  Ozeki, Haruo¹  Fukuzawa, Hideya²  Ohyama, Kanji²  Shirai, Hiromasa²  Inokucri, Hachiro¹  Kohchi, Takayuki² 

[1] Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606, Japan;>Laboratory of Plant Molecular Biology, Research Center for Cell and Tissue Culture, Faculty of Agriculture,Kyoto University, Kyoto 606, Japan

关键词: Chloroplast;    Ribosomal protein S12;    Intron;    Trans-splicing;    (Marchantia polymorpha);   

DOI  :  10.1016/0014-5793(86)81174-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

During the nucleotide sequencing of chloroplast DNA from the liverwort, M. polymorpha, we found that a coding sequence corresponding to the Esherichia coli ribosomal protein S12 gene (rps 12) is split into three exons. Strikingly, the first exon with the 5'-intron boundary sequence was found on the opposite strand of the chloroplast DNA (120 kilobases long, circular molecules) approx. 60 kilobases away from the rest of the exons. The amino acid sequence deduced from the DNA sequence was highly homologous to the sequences of the S12 ribosomal protein of E. coli (70.2%), and Euglena gracilis chloroplasts (73.6%). Possible mechanisms for the expression of this split gene are discussed.

【 授权许可】

Unknown