Primary structure of the α‐subunit of bovine adenylate cyclase‐inhibiting G‐protein deduced from the cDNA sequence

FEBS Letters

Tanabe, Tsutomu¹  Haga, Kazuko²  Nukada, Toshihide¹  Takahashi, Hideo¹  Ichiyama, Arata²  Kangawa, Kenji³  Noda, Masaharu¹  Numa, Shosaku¹  Haga, Tatsuya²  Hiranaga, Masuyo³  Matsuo, Hisayuki³ 

[1] Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606 Japan;Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu 431-31 Japan;Department of Biochemistry, Miyazaki Medical College, Miyazaki 889-16, Japan

关键词: Adenylate cyclase G-protein cDNA Cloning Nucleotide sequence Transducin ADP-ribosylation site;    G-protein;    guanine nucleotide-binding protein;    Gi;    adenylate cyclase-inhibiting G-protein;    Gs;    adenylate cyclase-stimulating G-protein;    Go;    a G-protein purified from brain;    HPLC;    high-performance liquid chromatography;    IAP;    islet-activating protein;   

DOI  :  10.1016/0014-5793(86)80347-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The primary structure of the α-subunit of the adenylate cyclase-inhibiting G-protein (g_i) has been deduced from the nucleotide sequence of cloned DNA complementary to the bovine cerebral mRNA encoding the polypeptide. A much higher degree of amino acid sequence homology is observed between the α-subunits of G_i and transducin (68%) than between those of G_i and the adenylate cyclase-stimulating G-protein (G_s) (43%) or between those of transucin and G_s (42%).

【 授权许可】

Unknown