Purification and properties of an oxygen‐evolving reaction center complex from photosystem II membranes

FEBS Letters

Yocum, Charles F.¹  Ghanotakis, Demetrios F.¹ 

[1] Division of Biological Sciences and Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1048, USA

关键词: Oxygen evolution Photosystem II Polypeptide Reaction center;    Chl;    chlorophyll;    DCBQ;    2;    5-dichloro-p-benzoquinone;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    LHC;    light-harvesting complex;    Mes;    4-morpholineethanesulfonic acid;    OOP;    1-O-n-octyl-β-D-glucopyranoside;    PS;    photosystem;    RC;    reaction center;   

DOI  :  10.1016/0014-5793(86)80335-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A method is reported for the isolation of a highly resolved oxygen-evolving photosystem II reaction center preparation. This preparation can be separated from the more complex photosystem II membranes isolated by the procedure of Berthold et al. [(1981) FEBS Lett. 134, 231-234] by use of octylglucopyranoside at elevated ionic strengths; the oxygen-evolving material can be collected by centrifugation at relatively low g values (40000 × g) in yields estimated to be more than 80%. This new preparation lacks the 17 and 23 kDa extrinsic polypeptides; addition of calcium and chloride produces activities approaching 1000 μmol O₂/h per mg chlorophyll. Although activity is maximal in the presence of 2,5-dichloro-β-benzoquinone, the response of activity to ferricyanide and 3-(3,4-dichlorophenyl)-1,1-dimethylurea indicates that the reducing side of photosystem II has been modified in this new oxygen-evolving reaction center preparation.

【 授权许可】

Unknown