Quantitation of plastoquinone‐9 in photosystem II reaction center particles

FEBS Letters

Diner, Bruce A.¹  de Vitry, Catherine¹  Carles, Christophe² 

[1] Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75005 Paris, France;Laboratoire de Biochimie et de Technologie Laitières CNRZ, 78350 Jouy-en-Josas, France

关键词: Photosystem II;    Plastoquinone-9;    Primary acceptor;    Secondary donor HPLC TLC;    PQ-9;    plastoquinone-9 (9 corresponds to the number of isoprenoids units);    PS;    photosystem;    RC;    reaction center;   

DOI  :  10.1016/0014-5793(86)80246-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Quantitative analysis of PQ-9 was performed on photosystem II reaction center particles isolated from Chlamydomonas reinhardtii. These particles show light-driven electron transfer from secondary donor Z to primary quinone acceptor Q_A. If both Z and Q_A were PQ-9, we would expect to find 2 PQ-9/RC. Quantitative determinations indicate an average of 1.16 ± 0.14 PQ-9/RC. As Q_A is not covalently bound to the RC (shown by Q_A reversible extraction and reconstitution) and as Q_A is known through optical spectroscopy to be a plastoquinone, we attribute the PQ-9 to Q_A and conclude that Z is not a PQH₂-9.

【 授权许可】

Unknown