L protein, encoded by psbL, restores normal functioning of the primary quinone acceptor, QA, in isolated D1/D2/CP47/Cytb‐559/I photosystem II reaction center core complex

FEBS Letters

Kitamura, Kohji¹  Toyoshima, Yoshinori¹  Ozawa, Shinichiro¹  Shiina, Takashi¹ 

[1]Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-nihonmatucho, Sakyo-ku, Kyoto 606-01, Japan

关键词: Photosystem II;    QA;    psbL;    L protein;    Plastoquinone-9;    Bis-Tris;    bis(2-hydroxyethyl)iminotris(hydroxymethyl)-methane;    Cytb-559;    cytochrome b 559;    DBMIB;    2;    5-dibromo-3-methyl-6-isopropyl-1;    4-benzoquinone;    DCPIP;    2;    6-dichlorophenol-indophenol;    DGDG;    digalactosyldiglyceride;    DM;    n-dodecyl-β-D-maltopyranoside;    DPC;    diphenylcarbazide;    MES;    2-(n-morpholino)-ethanesulfome acid;    OG;    n-octyl-β-D-glueopyranoside;    OTG;    n-octyl-β-D-thioglucopyranoside;    PQ-9;    plastoquinone-9;    PSII;    photosystem 11;    QA;    primary quinone acceptor of photosystem II;    QB;    secondary quinone acceptor of photosystem II;    RC;    reaction center complex of photosystem II;    SDS-PAGE;    SDS polyaerylamide gel electrophoresis;    Tricine;    tris(hydroxymethyl)methylglycine;   

DOI  :  10.1016/0014-5793(94)01089-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plastoquinone-9(PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-55911, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted into the isolated complex. PQ-9 reconstituted in the complex with the help of thylakoid lipids (digalactosyldiglyceride) did not function as Q_A by itself. However, PQ-9 simultaneously reconstituted with L protein and the thylakoid lipids successfully functioned as Q_A in the complex. Other proteins of PSII origin, such as CP43, H, K, nuclear encoded 4.1 and 5.0 kDa proteins, are unable to restore the Q_A activity in the complex.

【 授权许可】

Unknown