Activation of membrane phospholipase C by vasopressin

FEBS Letters

Balestre, Marie-Noëlle¹  Guillon, Gilles¹  Mouillac, Bernard¹  Devilliers, Ginette¹ 

[1] Centre CNRS-INSERM de Pharmacologie-Endocrinologie, Rue de la Cardonille, BP 5055, 34100 Montpellier, France

关键词: Inositol trisphosphate;    Vasopressin;    GTP-binding protein;    (WRK1 cell);    Phospholipase C Guanyl nucleotide;    IP1;    inositol monophosphate;    IP2;    inositol bisphosphate;    IP3;    inositol trisphosphate;    PI;    phosphatidylinositol;    PIP;    phosphatidylinositol 4-phosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    LVP;    lysine vasopressin;    GTPγS;    guanosine 5 ' -O-thiotriphosphate;    Gpp(NH)p;    guanyl-5'-yl imidodi-phosphate;    des-Gly9-d(CH2)5AVP;    desglycine9-[1-β-mercapto-β;    β-cyclopentamethylene propionic acid]-arginine vasopressin;   

DOI  :  10.1016/0014-5793(86)80232-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vasopressin stimulates the liberation of labelled inositol phosphate in partially purified plasma membranes prepared from myo-[³H]inositol prelabelled WRK1 cells. This stimulatory effect was very rapid (165% stimulation of inositol trisphosphate accumulation after a 10 s incubation period in the presence of 1 μM vasopressin), concentration dependent (EC₅₀= 12 nM) and was abolished by an antagonist of the vasopressor response to vasopressin. GTP, even at high concentrations (0.1 mM), did not increase inositol phosphate release: it was found to be absolutely necessary for hormonal stimulation of phospholipase C activity. Non-hydrolysable analogues of GTP may also stimulate this enzyme activity.

【 授权许可】

Unknown