cAMP mediated proteolysis of the catalytic subunit of cAMP‐dependent protein kinase

FEBS Letters

Hemmings, Brian A.¹ 

[1] Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland

关键词: cyclic AMP;    cyclic AMP dependence;    Protein kinase;    Hormone action;    Proteolysis;   

DOI  :  10.1016/0014-5793(86)80226-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cAMP-dependent protein kinase from LLC-PK₁ cells can be activated in vivo by calcitonin and vasopressin, or forskolin. Continuous treatment of cells with these agents results in a decrease of total cAMP-PK activity. The loss of kinase activity was enhanced when either of these three agents was incubated in the presence of isobutylmethylxanthine. Results obtained using affinity purified antibodies to the catalytic subunit show that the loss of kinase was due to specific proteolysis of this subunit.

【 授权许可】

Unknown