Direct evidence for a coupling between synthesis and export of PhoS in E. coli

FEBS Letters

Pages, Jean-Marie¹  Lazdunski, Claude¹  Anba, Jamila¹ 

[1] Centre de Biochimie et de Biologie Moléculaire du CNRS, 31 Chemin Joseph Aiguier, BP 71, 13402 Marseille Cedex 9, France

关键词: Protein synthesis;    Protein export;    Periplasmic protein;    Signal sequence;    Signal recognition particle Pho regulon;   

DOI  :  10.1016/0014-5793(86)80204-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The accumulation of pre-PhoS under conditions of PhoS overproduction has been previously described. It is now demonstrated that during the induction of PhoS, a delay in the completion of polypeptide chain elongation can be detected. This delay is related to the extent of jamming of export sites by pre-PhoS or by other exported proteins. These results suggest that a component required for completion of pre-PhoS polypeptide becomes limiting, being titrated by the excess of nascent chains bearing signal peptides. This component thus probably acts at an early step in the export pathway.

【 授权许可】

Unknown