Export and secretion of overproduced OmpA‐β‐lactamase in Escherichia coli

FEBS Letters

Inouye, Masayori¹  Lazdunski, Claude²  Pagès, Jean-Marie²  Bolla, Jean-Michel² 

[1] Department of Biochemistry, University of Medicine & Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854-5635, USA;Centre de Biochimie et de Biologie Moléculaire du CNRS 31 Chemin Joseph Aiguier, BP 71, 13402 Marseille Cedex 9, France

关键词: Hybrid protein;    Protein export;    Signal peptide;    Protein processing;    Periplasmic protein;   

DOI  :  10.1016/0014-5793(87)80450-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The export of β-lactamase to the periplasm of Escherichia coli can be directed by the OmpA signal peptide in the secretion cloning vector pIN-III. The overproduction of the hybrid precursor specifically induces a delay in the onset of processing of newly synthesized polypeptide chains. However, when the processing starts, no alteration in the rate of cleavage itself is observed. Our results suggest that the temporal mode of processing (which reflects translocation) does not depend on the nature of the signal peptide but rather depends on the nature of the polypeptide chain exported.

【 授权许可】

Unknown