Amino acid substitutions in mitochondrial ATPase subunit 9 of saccharomyces cerevisiae leading to oligomycin or venturicidin resistance

FEBS Letters

Nagley, Phillip¹  Ooi, Beng Guat¹  Hall, Ruth M.¹ 

[1] Department of Biochemistry, and Centre for Molecular Biology and Medicine, Monash University, Clayton, Victoria 3168, Australia

关键词: Proteolipid Mitochondrial ATPase complex Amino acid substitution Cytoduction (Yeast) Drug-resistance domain Oligomycin Venturicidin;    DCCD;    N;    N'-dicyclohexylcarbodiimide;    mtATPase;    mitochondrial proton-translocating ATPase;    oliR;    oligomycin-resistant (in reference to phenotype);    TID;    3-(trifluoromethyl)-3-(m-[125I]-phenyl)diazirin;   

DOI  :  10.1016/0014-5793(86)80152-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A series of isonuclear oligomycin-resistant mutants of Saccharomyces cerevisiae carrying mutations in the mitochondrial olil gene has been studied. DNA sequence analysis of this gene has been used to define the amino acid substitutions in subunit 9 of the mitochondrial ATPase complex. A domain of amino acids involved in oligomycin resistance can be recognized which encompasses residues in each of the two hydrophobic portions of the subunit 9 polypeptide that are thought to span the inner mitochondrial membrane. Certain amino acid substitutions also confer cross-resistance to venturicidin: these residues define an inner domain for venturicidin resistance. The expression of venturicidin resistance resulting from one particular substitution is modulated by nuclear genetic factors.

【 授权许可】

Unknown