Nonenzymatic radiolabeling of protein by 32P‐containing nucleotides

FEBS Letters

Hanna, Michelle M.¹  Schmidt, Martin C.¹ 

[1] Department of Biochemistry, University of California, Berkeley, CA 94720, USA

关键词: Nonenzymatic protein phosphorylation;    Protein radiolabeling;    Photoaffinity labeling;    nusA protein;   

DOI  :  10.1016/0014-5793(86)80106-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a nonenzymatic reaction which results in the radiolabeling of proteins by ³²P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modification. This nonspecific labeling represents a serious artifact which may become significant in systems involving low levels of specific labeling, such as photoaffinity labeling. Since the reaction is not limited to specific proteins, this may, however, provide a simple and rapid procedure for the preparation of labeled proteins.

【 授权许可】

Unknown