Hydrophobic photolabelling of pertussis toxin subunits interacting with lipids

FEBS Letters

Montecucco, Cesare¹  Tomasi, Maurizio²  Schiavo, Giampietro¹  Rappuoli, Rino³ 

[1] Centra CNR Fisiologia Mitocondri e Istituto di Patologia Generale, Università di Padova, Via Loredan 16, 35131 PadovaItaly;Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 RomaItaly;Sclavo Research Center, Via Fiorentina 1, 53100 Siena, Italy

关键词: Pertussis toxin;    Hydrophobic interaction;    Photolabeling;   

DOI  :  10.1016/0014-5793(86)80105-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hydrophobic surfaces presumably involved in the membrane interaction of pertussis toxin have been mapped by a new detergent-binding assay. This is based on the interdispersion among detergent micelles of trace amounts of radioactive photoreactive phospholipid analogues, able to cross-link to the protein thereby labelling its detergent-binding domains. The assay has proven to be very sensitive. Subunits B₁, B₂ and B₃ of pertussis toxin were found to interact with the lipid micelles suggesting that they may be involved in the membrane penetration step of the intoxication process.

【 授权许可】

Unknown